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Image Search Results
Journal: Nature immunology
Article Title: Foxp3 and Toll-like receptor signaling balance T reg cell anabolic metabolism for suppression
doi: 10.1038/ni.3577
Figure Lengend Snippet: Foxp3 reduces aerobic glycolysis and promotes mitochondrial oxidative metabolism. (a) Gene-expression microarray analysis and pathway analysis (with the DAVID bioinformatics database) of RNA from primary mouse CD4+CD25− T cells stimulated and transduced with control or Foxp3-expressing retrovirus, presented as enrichment for selected pathways (left and right margins) among genes upregulated (top) or downregulated (bottom) by Foxp3. (b–g) Immunoblot analysis (b,c), glucose uptake (d), glycolysis (as ECAR after glucose injection) (e) basal OCR (f) and basal OCR/ECAR (g) of primary mouse CD4+CD25− T cells stimulated and transduced with control (NGFR) or Foxp3-expressing retrovirus. Numbers above lanes (b,c) indicate band intensity relative to that of actin. CPM, counts per minute. *P < 0.05 (Two-tailed Student’s t-test). Data are representative of an experiment with biological triplicates for each group (a), three independent experiments (b,c) or two independent experiments with technical triplicates (d–g; mean + s.d.).
Article Snippet: The cDNA was used in the
Techniques: Gene Expression, Microarray, Transduction, Control, Expressing, Western Blot, Injection, Two Tailed Test
Journal: Nature immunology
Article Title: Foxp3 and Toll-like receptor signaling balance T reg cell anabolic metabolism for suppression
doi: 10.1038/ni.3577
Figure Lengend Snippet: Foxp3 expression is sufficient to suppress glycolysis and promote oxidative metabolism. (a–i) Glucose uptake (a), glycolytic rate (assessed by a radiolabeled-glucose-tracer assay) (b), ECAR (c), flux of glucose into the pentose phosphate pathway (PPP) (d), OCR (e), OCR/ECAR (f), pyruvate oxidation (g), fatty-acid oxidation of palmitate (h) and glutamine oxidation (i) of vector-control-transduced FL5.12 pro-B cells or FL5.12 pro-B cells transduced with tamoxifen-inducible expression of Foxp3, treated with 4-hydroxytamoxifen. (j) Quantification of live cells as in a–i to assess cell accumulation over time. *P < 0.05 (two-tailed Student’s t test). Data are representative of three (a,b,j) or two (c–i) independent experiments with three independent clones per group (mean + s.d. in a–i or (mean ± s.d. in j).
Article Snippet: The cDNA was used in the
Techniques: Expressing, Plasmid Preparation, Control, Transduction, Two Tailed Test, Clone Assay